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Ham‘s F10 Medium Print

DESCRIPTION

Ham‘s F10 is an alternative of Ham‘s F12 and was used primarily to culture CHO-cells. Today Ham‘s F10 can be used with or without FBS for many different cells cultures. It finds a use for example in primary cells of rat and chicken, but also for human diploid cells.

 

 

LIQUID MEDIA

Ham's F10 Medium 500 ml P04-12050
without L-Glutamine
with 1,2 g/l NaHCO3
-
Ham's F10 Medium 500 ml P04-12500
with L-Glutamine
with 1,2 g/l NaHCO3
-
Ham's F10 Medium 500 ml P04-13500
without stab. Glutamine
with 1,2 g/l NaHCO3
-
Ham's F10 Medium 500 ml P04-13050
with L-Glutamine
with 25 mM Hepes
with 1,2 g/l NaHCO3
-
Ham's F10 Medium 500 ml P04-12049
without L-Glutamine
without Phenol red
with 1,2 g/l NaHCO3
-
Ham's F10 Medium 500 ml P04-13501
without stab. Glutamine
without Glucose
with 1,2 g/l NaHCO3
-
Ham's F10 Medium 500 ml P04-13550
without stab. Glutamine
without Phenol red
with 1,2 g/l NaHCO3

 

 

 

POWDER MEDIA

Ham's F-10 Medium 10 L P03-5010
without L-Glutamine 50 L P03-5050
without NaHCO3
-
Ham's F-10 Medium 10 L P03-3910
with L-Glutamine 50 L P03-3950
without NaHCO3
-
Ham's F-10 Medium 10 L P03-4010
with L-Glutamine 50 L P03-4050
with 25 mM Hepes
without NaHCO3

 

 

 

COMPOSITION

Components mg/L
Inorganic Salts Calcium chloride x 2H2O 44,09
Copper(II) sulfate x 5H2O 0,003
Iron(II) sulfate x 7H2O 0,834
Magnesium sulfate dried 74,62
Potassium chloride 285,00
Potassium dihydrogen phosphate 83,00
Sodium chloride 7400,00
di-Sodium hydrogen phosphate anhydrous 153,70
Zinc sulfate x 7H2O 0,029
Other Components D(+)-Glucose anhydrous 1100,00
Hypoxanthine 4,08
DL-α-Lipoic acid 0,21
Hepes 5958,00
Phenol red 1,20
Sodium pyruvate 110,00
2`-Deoxythymidine 0,73
Amino acids L-Alanine 8,91
L-Arginine x HCl 211,00
L-Asparagine x H2O 15,00
L-Aspartic acid 13,30
L-Cysteine x HCl x H2O 35,12
L-Glutamine 146,20
L-Glutamic acid 14,70
Glycine 7,51
L-Histidine x HCl x H2O 21,00
L-Isoleucine 2,60
L-Leucine 13,10
L-Lysine x HCl 29,30
L-Methionine 4,48
L-Phenylalanine 4,96
L-Proline 11,50
L-Serine 10,50
L-Threonine 3,57
L-Tryptophan 0,60
L-Tyrosine 1,81
L-Valine 3,50
Vitamins D(+)-Biotin 0,024
D-Calcium pantothenate 0,715
Choline chloride 0,698
Folic acid 1,32
myo-Inositol 0,541
Nicotinamide 0,615
Pyridoxine x HCl 0,21
Riboflavin 0,376
Thiamine x HCl 1,01
Vitamin B12 1,36

When 5958,00 mg/l Hepes are included there are only 6900 mg /l Sodium chloride.

 

 

 

SCHEDULE

L-Glutamine Stab. Glutamine Phenol red Hepes Special
12050 x
12500 x x
13500 x x
13050 x x 25 mM
12049
13501 x x
13550 x

 

 

 

Each formulation possible!