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Ham‘s F12 Medium Print

DESCRIPTION

In the past Ham‘s F12 was the first choice for a serumfree cultivation of CHO-cells and is now substituted through better serumfree systems like our C6000, which is also proteinfree. However it is an appropriate medium for mammalian cells when it is supplemented with FBS. It contains a high concentration of vitamins, amino acids and trace elements. The content of zinc sulphate is increased and it contains putrescine and linoleic acid.

 

 

 

LIQUID MEDIA

Ham’s F12 Medium 500 ml P04-14550
without L-Glutamine
with 1,176 g/l NaHCO3
-
Ham’s F12 Medium 500 ml P04-14500
with L-Glutamine
with 1,176 g/l NaHCO3
-
Ham’s F12 Medium 500 ml P04-14501
with L-Glutamine
without Phenol red
with 25 mM Hepes
with 1,176 g/l NaHCO3
-
Ham’s F12 Medium 500 ml P04-15500
with stab. Glutamine
with 1,176 g/l NaHCO3
-
Ham’s F12 Medium 500 ml P04-14559
without L-Glutamine
without Phenol red
with 1,176 g/l NaHCO3
-
Ham’s F12 Medium 500 ml P04-15600
with L-Glutamine
with 2,5 g/l NaHCO3

 

 

 

SPECIAL MEDIA

Minimum order quantity: 20 x 500 ml

Hams’s F12 Medium 500 ml P04-15700
without L-Glutamine
with 2,5 g/l NaHCO3
-
Hams’s F12 Medium 500 ml P04-15502
with stab. Glutamine
without Methionine
without Cysteine
with 1,176 g/l NaHCO3
-
Ham’s F12/IMDM (1:1) 500 ml P04-20950
without L-Glutamine
with 2,1 g/l NaHCO3
supplemented with:
7,5 % Foetal Bovine Serum
7,5 % Newborn Calfserum
52,6 mg/l D-Valine
Antibiotics/Antimyotcs admixture
-
Ham’s F12 Medium 500 ml P04-15549
with stab. Glutamine
without Folic acid
with 1,176 g/l NaHCO3
-
Ham’s F12 Medium 500 ml P04-15548
without L-Glutamine
without Folic acid
with 1,176 g/l NaHCO3

 

 

 

POWDER MEDIA

Ham’s F12 Medium 10 L P03-4910
without L-Glutamine 50 L P03-4950
without NaHCO3
-
Ham’s F12 Medium 10 L P03-4110
with L-Glutamine 50 L P03-4150
without NaHCO3
-
Ham’s F12 Medium 10 L P03-4210
with L-Glutamine 50 L P03-4250
with 25 mM Hepes
without NaHCO3

 

 

 

SPECIAL MEDIA

Minimum order quantity: 20 x 500 ml

Ham’s F12/DMEM (1:3) 10 L P03-9910
with L-Glutamine 50 L P03-9950
without Phenol red
with 7,5 mM Hepes
with 2,075 mg/l Glucose
without NaHCO3

 

 

 

COMPOSITION

Components mg/L
Inorganic Salts Calcium chloride anhydrous 33,30
Copper(II) sulfate x 5H2O 0,003
Iron(II) sulfate x 7H2O 0,834
Magnesium chloride x 6H2O 122,00
Potassium chloride 223,60
Sodium chloride 7099,00
di-Sodium hydrogen phosphate anhydrous 142,04
Zinc sulfate x 7H2O 0,86
Other Components D(+)-Glucose anhydrous 1801,60
Hepes 5958,00
Hypoxanthine 5,46
Linoleic acid 0,084
DL-α-Lipoic acid 0,21
Phenol red 1,20
Putrescine x 2HCl 0,16
Sodium pyruvate 110,10
Thymidine 0,73
Amino acids L-Alanine 8,91
L-Arginine x HCl 210,70
L-Asparagine x H2O 15,01
L-Aspartic acid 13,31
L-Cysteine x HCl x H2O 35,12
L-Glutamine 146,20
L-Glutamic acid 14,71
Glycine 7,51
L-Histidine x HCl x H2O 20,96
L-Isoleucine 3,94
L-Leucine 13,12
L-Lysine x HCl 36,54
L-Methionine 4,48
L-Phenylalanine 4,96
L-Proline 34,53
L-Serine 10,51
L-Threonine 11,91
L-Tryptophan 2,04
L-Tyrosine x 2Na x 2H2O 7,84
L-Valine 11,71
Vitamins D(+)-Biotin 0,007
D-Calcium pantothenate 0,24
Choline chloride 13,96
Folic acid 1,32
myo-Inositol 18,02
Nicotinamide 0,037
Pyridoxine x HCl 0,062
Riboflavin 0,038
Thiamine x HCl 0,34
Vitamin B12 1,36

 

 

 

SCHEDULE

L-Glutamine Stab. Glutamine Sodium pyruvate Phenol red Hepes Special
14550 x x
14500 x x x
14501 x 25 mM
15500 x x x
14559 x
15600 x x x s.o.
15700 x x s.o.
15502 x x x s.o.
15549 x x x s.o.
15548 x x s.o.
20950 x x s.o.

 

 

 

Each formulation possible!