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DESCRIPTION
The CMRL is a Nucleosid- and Vitamin-rich Medium. In the past it was developed to clone monkey-kidney cells and as long time culture medium for L-cells. It is suitable for many types of human and monkey cells and also for other mammalian cells, especially by using horse and calf serum.
LIQUID MEDIA
| CMRL – 1066 |
500 ml |
P04-84600 |
| without L-Glutamine |
|
|
| without Phenol red |
|
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| with 2,2 g/l NaHCO3 |
|
|
| - |
|
|
| CMRL – 1066 |
500 ml |
P04-84500 |
| with L-Glutamine |
|
|
| without Phenol red |
|
|
| with 2,2 g/l NaHCO3 |
|
|
POWDER MEDIA
| CRML – 1066 |
10 L |
P03-8410 |
| without Glutamine |
50 L |
P03-8450 |
| without Phenol red |
|
|
| without NaHCO3 |
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COMPOSITION
|
Components |
mg/L |
| Inorganic Salts |
Calcium chloride x 2H2O |
264,92 |
|
Potassium chloride |
400,00 |
|
Magnesium anhydros |
97,78 |
|
Sodium acetate x 3H2O |
83,00 |
|
Sodium chloride |
6799,00 |
|
di-Sodium hydrogen phosphate x 2H2O |
139,57 |
| Other Components |
Cholesterol |
0,20 |
|
D(+)-Glucose anhydrous |
1000,00 |
|
Glutathione (red.) |
10,00 |
|
5-Methyldeoxycytidine |
0,10 |
|
Sodium glucuronate x H2O |
4,00 |
|
Tween 80 |
5,00 |
| Coenzyme |
Cocarboxylase x HCl |
1,00 |
|
Coenzym A Trilithiumsalt x 2H2O |
2,67 |
|
FAD |
1,00 |
|
NAD |
7,00 |
|
NADP |
1,00 |
|
UTP |
1,00 |
| Amino acids |
L-Alanine |
25,00 |
|
L-Arginine x HCl |
70,00 |
|
L-Aspartic acid |
30,00 |
|
L-Cysteine free base |
199,66 |
|
L-Cystine |
20,00 |
|
L-Glutamine |
100,00 |
|
L-Glutamic acid |
75,00 |
|
Glycine |
50,00 |
|
L-Histidine x HCl x H2O |
20,00 |
|
L-Hydroxyproline |
10,00 |
|
L-Isoleucine |
20,00 |
|
L-Leucine |
60,00 |
|
L-Lysine x HCl |
70,00 |
|
L-Methionine |
15,00 |
|
L-Phenylalanine |
25,00 |
|
L-Proline |
40,00 |
|
L-Serine |
25,00 |
|
L-Threonine |
30,00 |
|
L-Tryptophan |
10,00 |
|
L-Tyrosine |
40,00 |
|
L-Valine |
25,00 |
| Vitamins |
p-Aminobenzioc acid |
0,05 |
|
L-Ascorbin acid |
50,00 |
|
D(+)-Biotine |
0,01 |
|
D-Calcium pantothenate |
0,01 |
|
Choline chloride |
0,50 |
|
Folic acid |
0,01 |
|
myo-Inositol |
0,05 |
|
Nicotinic acid |
0,025 |
|
Nicotinamide |
0,025 |
|
Pyridoxal x HCl |
0,025 |
|
Pyridoxide x HCl |
0,025 |
|
Riboflavin |
0,01 |
|
Thiamine x HCl |
0,01 |
| Desoxyribonucleoside |
2´- Deoxyadenosine |
10,00 |
|
2´- Deoxycytidine x HCl |
11,60 |
|
2´- Deoxyguanosine |
10,00 |
|
2´- Deoxythymidine |
10,00 |
Each formulation possible!
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