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BESCHREIBUNG
Mitte der 50er Jahre erkannte man, dass Säugetierzellen neben den zehn essentiellen Aminosäuren auch Cystin, Tyrosin und Glutamin benötigen. Neben diesen drei Aminosäuren enthält die Formulierung des BME auch B-Vitamine. Ursprünglich gedacht für die Kultivierung von Maus-L-Zellen und Hela-Zellen, findet BME heute mit seinen vielen Variationen weite Verbreitung. Neben der Kultivierung von normalen Säugetierzellen eignet sich das BME auch sehr gut für transformierte Zellen.
FLÜSSIGMEDIEN
| BME with EBSS |
500 ml |
P04-25050 |
| without L-Glutamine |
|
|
| with 2,2 g/l NaHCO3 |
|
|
| -- |
|
|
| BME with EBSS |
500 ml |
P04-25500 |
| with L-Glutamine |
|
|
| with 2,2 g/l NaHCO3 |
|
|
| -- |
|
|
| BME with EBSS |
500 ml |
P04-25151 |
| with stab. Glutamine |
|
|
| with 2,2 g/l NaHCO3 |
|
|
| -- |
|
|
| BME with EBSS |
500 ml |
P04-25350 |
| with L-Glutamine |
|
|
| without Phenol red |
|
|
| with 2,2 g/l NaHCO3 |
|
|
| -- |
|
|
| BME with EBSS (2x) |
50 ml |
P04-21017 |
| with L-Glutamine |
|
|
| without Phenol red |
|
|
| with 4,4 g/l NaHCO3 |
|
|
| -- |
|
|
| BME with EBSS |
500 ml |
P04-25150 |
| with stab. Glutamine |
|
|
| without NaHCO3 |
|
|
| -- |
|
|
| BME with EBSS |
500 ml |
P04-25250 |
| without L-Glutamine |
|
|
| with 25 mM Hepes |
|
|
| with 2,2 g/l NaHCO3 |
|
|
PULVERMEDIEN
| BME with EBSS |
10 L |
P03-5610 |
| without L-Glutamine |
50 L |
P03-5650 |
| without NaHCO3 |
|
|
| -- |
|
|
| BME with EBSS |
10 L |
P03-0110 |
| with L-Glutamine |
50 L |
P03-0150 |
| without NaHCO3 |
|
|
| -- |
|
|
| BME with EBSS |
10 L |
P03-5710 |
| without L-Glutamine |
50 L |
P03-5750 |
| with 25 mM Hepes |
|
|
| without NaHCO3 |
|
|
| -- |
|
|
| BME with EBSS |
10 L |
P03-0210 |
| with L-Glutamine |
50 L |
P03-0250 |
| with 25 mM Hepes |
|
|
| without NaHCO3 |
|
|
ZUSAMMENSETZUNG
|
Components |
mg/L |
| Inorganic Salts |
Calcium chloride x 2H2O |
264,92 |
|
Magnesium sulfate dried |
139,52 |
|
Potassium chloride |
400,00 |
|
Sodium chloride |
6800,00 |
|
Sodium dihydrogen phosphate x H2O |
140,00 |
| Other Components |
D(+)-Glucose anhydrous |
1000,00 |
|
Hepes |
5958,00 |
|
Phenol red |
10,00 |
| Amino acids |
L-Arginine x HCl |
21,00 |
|
L-Cystine |
12,00 |
|
L-Glutamine |
292,00 |
|
L-Histidine Base |
8,00 |
|
L-Isoleucine |
26,00 |
|
L-Leucine |
26,00 |
|
L-Lysine x HCl |
36,47 |
|
L-Methionine |
7,50 |
|
L-Phenylalanine |
16,50 |
|
L-Threonine |
24,00 |
|
L-Tryptophan |
4,00 |
|
L-Tyrosine |
18,00 |
|
L-Valine |
23,50 |
| Vitamins |
D(+)-Biotin |
1,00 |
|
D-Calcium pantothenate |
1,00 |
|
Choline chloride |
1,00 |
|
Folic acid |
1,00 |
|
myo-Inositol |
2,00 |
|
Nicotinamide |
1,00 |
|
Pyridoxal x HCl |
1,00 |
|
Riboflavin |
0,10 |
|
Thiamine x HCl |
1,00 |
Wenn enthalten 5958,00 mg/l Hepes sind, sind nur 6300 mg /l Natriumchlorid enthalten.
TABELLE
|
L-Glutamine |
Stab. Glutamine |
Phenolred |
Hepes |
Special |
| 25050 |
|
|
x |
|
|
| 25500 |
x |
|
x |
|
|
| 25151 |
|
x |
x |
|
|
| 25350 |
x |
|
|
|
|
| 21017 |
x |
|
|
|
s.o. |
| 25150 |
|
x |
x |
|
s.o. |
| 25250 |
|
|
x |
25 mM |
|
Jede abweichende Formulierung möglich!
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