New Technologies

In series of tests for many years PAN-Biotech has developed, optimized and successfully marketed a number of serumfree media for many different cell types. The development and optimization of serumfree media takes a lot of time and expenditure. Several months or even years can often pass until the release of a new product.

With the development of a fully-automated cell culture System –  he new PANsys3000 – PAN-Biotech pursues a completely different technology. With this system we have succeeded in cultivating different samples simultaneously under identical conditions and under constant microscopic observation. By adding individual components res. by changing the concentration, a positive (improvement of growth) res. negative effect (inhibition of growth) can directly be measured. All relevant data can be stored and recalled later. Morphologic changes are identified immediately and evaluated depending on the media composition.



PANsys 3000 - The World‘s first fully-automated Cell Culture System

More and detailled information to the automated cell culture system PANsys3000 is available here :

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The automated cell culture system offers unique features and many advantages for the development of serumfree media:

• Reduction of the development periods by more convincing results.
• Adjusting the optimal concentration of individual substances.
• Comparison of the measurements of competitor products and recording the results.
• Culture conditions (temperature, CO2-fumigation) can be continuously changed at any time.
• Complete control of individual batches.

In order to satisfy our customers’ demands, we will continue to expand and optimize our range of serum- and proteinfree media.

Beside the „ready-for-use“ nutrient solutions (PANSERINSeries) we can also offer different types of serum substitutes (PANEXIN-Series) to our customers which are added in a concentration of 2 % - 8 % instead of the serum to the respective basic media (e.g. RPMI 1640, DMEM, DMEM/F12).

In the following example, CHO-cells were cultivated in DMEM with 4,5 g/l glucose with L-glutamine, with pyruvate and different serum substitutes (seed 1 x 104/ml, evaluation 7th day, cells not adapted).

CHO with serum substitute 1 Cells do not form any cell layer, loose attachment	CHO with serum substitute 2 Cells do not form any cell layer, loose attachment	CHO with 5 % PANEXIN Cells form uniform cell layer

Serumfree nutrient media without animal or human components res. proteinfree media are also already available from PAN-Biotech.

Due to the modern and unique technology for the development of serumfree media of the 2nd-Generation in many cases an adaption of the cells to the serumfree culture is not necessary; with critical cells (primary cells) the cells slowly adapt to the serumfree conditions with the help of our detailed records and protocols. We will be pleased to support you with our expertise.