|
DESCRIPTION
Features and applications
- Ultra-pure: > 99 % trisphosphate by HPLC
- Extended shelf-life of 24 months at -20° C
- Free from PCR inhibitors
- DNase, RNase and Nickase free
- Manufactured by Bioline in a purpose-built facility
Suitable for a wide variety of applications such as:
- Standard and long range PCR assays
- cDNA synthesis
- qPCR
- Microarrays
- DNA sequencing
- DHPLC
- Labeling
A set of ready-to-use molecular grade dNTP solutions consisting of 4 separate 100mM solutions of dATP, dGTP, dCTP and
dTTP, at pH 7.5 and supplied as lithium salts in purified water. For use in DNA polymerization reactions, DNA labeling and
sequencing processes. Dependable PCR grade.
All dNTPs are supplied as Lithium salts in purified water at pH 7.5. Lithium salts have greater resistance to repeated
freezing and thawing cycles than Sodium salts, and Lithium salt dNTP preparations remain sterile over the entire shelflife
due to the bacteriostatic activity of Lithium towards various microorganisms.
Storage Conditions
dNTP Set can be stored for 24 months at -20° C. Avoid multiple freeze/thaw cycles.
For long-term storage, aliquoting is recommended.
| Caracteristics |
dATP |
dCTP |
dGTP |
dTTP |
| Product |
dATP Lithium
100 mM Solution |
dCTP Lithium
100 mM Solution |
dGTP Lithium
100 mM Solution |
dTTP Lithium
100 mM Solution |
| Nomenclature |
2'-deoxyadenosine-
5'-triphosphate |
2'-deoxyadenosine-
5'-triphosphate |
2'-deoxyadenosine-
5'-triphosphate |
2'-deoxyadenosine-
5'-triphosphate |
| Formula |
C10H12N5O12P3Li4 |
C9H12N3O13P3Li4 |
C10H12N5O13P3Li4 |
C10H13N2O14P3Li4 |
| Molecular Weight |
514.9 g/mol |
490.9 g/mol |
530.9 g/mol |
505.9 g/mol |
| λmax pH 7.0 |
259 nm |
272 nm |
252 nm |
267 nm |
| ε at λmax @ pH7.0 |
15.4 E x mmol-1 x cm-1 |
9,1 E x mmol-1 x cm-1 |
13.7 E x mmol-1 x cm-1 |
9.6 E x mmol-1 x cm-1 |
| A250/A260 |
0.78 ± 0.03 |
0.82 ± 0.03 |
1.16 ± 0.05 |
0.65 ± 0.03 |
| A280/A260 |
0.15 ± 0.02 |
0.98 ± 0.03 |
0.66 ± 0.03 |
0.73 ± 0.02 |
| Concentration |
100mM ± 2% |
100mM ± 2% |
100mM ± 2% |
100mM ± 2% |
| Appearance |
Clear Colorless
Solution |
Clear Colorless
Solution |
Clear Colorless
Solution |
Clear Colorless
Solution |
| pH of Solution |
7.5 |
7.5 |
7.5 |
7.5 |
| dNTP (HPLC Area) |
≥ 99 % |
≥ 99 % |
≥ 99 % |
≥ 99 % |
| dNDP (HPLC Area) |
< 1 % |
< 1 % |
< 1 % |
< 1 % |
DNases, RNases,
Nicking Activity |
Negative |
Negative |
Negative |
Negative |
| Storage |
at -20° C |
at -20° C |
at -20° C |
at -20° C |
| Stability |
≤ 24 months |
≤ 24 months |
≤ 24 months |
≤ 24 months |
| dNTP set (dA, dC, dG, and dT) |
100 mM |
4 x 250 μl |
PAN739025 |
|
100 mM |
4 x (4 x 250 μl) |
PAN739026 |
|
100 mM |
4 x (20 x 250 μl) |
PAN739027 |
DESCRIPTION
Features and applications
- Convenient, pre-optimized and pre-mixed
- Ultra-pure: > 99 % trisphosphate by HPLC
- Extended shelf-life of 24 months at -20° C
- Free from PCR inhibitors
- DNase, RNase and Nickase free
- Manufactured by Bioline in a purpose-built facility
Suitable for a wide variety of applications such as:
- Standard and long range PCR assays
- cDNA synthesis
- qPCR
- Microarrays
- DNA sequencing
- DHPLC
- Labeling
A ready-to-use molecular grade dNTP Mix containing dATP, dCTP, dGTP and dTTP at pH 7.5 as Lithium salts in purified water. The mix is designed to save hands-on time for researchers and minimize the possibility of contamination. For use in DNA polymerization reactions, DNA labeling and sequencing processes. Dependable PCR grade.
All dNTPs are supplied as Lithium salts in purified water at pH 7.5. Lithium salts have greater resistance to repeated freezing and thawing cycles than Sodium salts, and Lithium salt dNTP preparations remain sterile over the entire shelflife due to the bacteriostatic activity of Lithium towards various microorganisms.
dNTP Mix Reaction Guidelines
100 mM Mix contains 25 mM of each dNTP
Reaction Volume Master Mix Reactions
50 μl 0.5 μl 1000
40 mM Mix contains 10 mM of each dNTP
Reaction Volume Master Mix Reactions
50 μl 1.25 μl 400
This is a guide only, for long-range applications adjust accordingly.
Storage Conditions
dNTP Mix can be stored for 24 months at -20° C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended.
Typical Analysis
Lithium salts, > 99 % deoxynucleoside triphosphates (HPLC, area %), < 1 % deoxynucleoside monophosphates and deoxynucleoside diphosphates.
Purity
The dNTPs are > 99 % pure by HPLC and are free of DNase, RNase, Protease, phosphatase and nicking activity.
|
20 μmol |
40 mM |
500 μl |
PAN739043 |
|
50 μmol |
100 mM |
500 μl |
PAN739028 |
| dNTP Mix (dA + dC + dG + dT) |
200 μmol |
100 mM |
4 x 500 μl |
PAN739029 |
DESCRIPTION
Features and applications
- > 99 % pure by HPLC
- Extended shelf-life of 24 months at -20° C
- Free from PCR inhibitors
- DNase, RNase and Nickase free
- Manufactured by Bioline in a purpose-built laboratory
- Custom, Bulk and OEM Nucleotides service
Suitable for a wide variety of applications such as:
- Standard PCR assays
- Long range PCR assays
- cDNA synthesis
- qPCR
- Microarrays
- DNA sequencing
- Mutagenesis
- Genotyping
- DHPLC
- Labeling
Ultra-pure dNTPs are enzymatically synthesized from premium quality raw materials, using highly specific production systems in our purpose built facilities. The manufacturing process eliminates impurities and PCR-specific inhibitors such as modified nucleotides, tetraphosphates and pyrophosphates commonly observed in other commercially available dNTP products. The dNTPs are purified with quantitative HPLC and possess at least 99 % purity.
All dNTPs are supplied as Lithium salts in purified water at pH 7.5. Lithium salts have greater resistance to repeated freezing and thawing cycles than Sodium salts, and Lithium salt dNTP preparations remain sterile over the entire shelflife due to the bacteriostatic activity of Lithium towards various microorganisms.
Storage and Stability
dATP can be stored for 24 months at -20° C or -70° C in a constant temperature freezer. Avoid multiple freezing/thawing. For long-term usage, aliquoting is recommended.
| dATP |
100 mM as 1 x 250 μl |
25 μmol |
PAN739036 |
| dCTP |
100 mM as 1 x 250 μl |
25 μmol |
PAN739038 |
| dGTP |
100 mM as 1 x 250 μl |
25 μmol |
PAN739037 |
| dTTP |
100 mM as 1 x 250 μl |
25 μmol |
PAN739039 |
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