| ADDITIVES AND BUFFERS |
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DESCRIPTION Applications
Storage Conditions 10x NH4 Buffer can be stored for 12 months at -20° C. Avoid multiple freeze/thaw cycles. Composition (10x) 160 mM (NH4)2SO4, 670 mM Tris-HCl (pH 8.8 at 25° C) and stabilizer.
DESCRIPTION 50mM MgCl2 is a convenient concentration for most molecular experiments. Use 1 μl in a 50 μl reaction for a final Mg2+ concentration of 1mM. Storage Conditions 50 mM MgCl2 Solution should be stored at -20° C. Avoid multiple freeze/thaw cycles. Composition MgCl2 50 mM in water, DNase/RNase-free
DESCRIPTION 10x KCl Buffer provides reliable performance with the convenience of 15 mM MgCl2, which is suitable for most applications. This makes it ideal for high-throughput experiments. Storage Conditions 10x KCl Reaction Buffer should be stored at -20° C. Avoid multiple freeze/thaw cycles. Composition 500 mM KCl, 100 mM Tris-CI (pH 8.8 at 25° C), 15 mM MgCl2, 1 % Triton X-100 15 mM.
DESCRIPTION Features and applications
HiSpec additive is a popular compound designed to eliminate unwanted byproducts, such as background smears and spurious bands, during DNA amplification. Hi-Spec Additive is ideally suited to difficult templates with GC-rich regions or repetitive sequences. Storage Conditions HiSpec additive can be stored for 6 months at -20° C.
DESCRIPTION Features and applications
Opti Buffer is a non-Tris incubation buffer for all polymerase applications where optimal conditions are essential to achieve a high yield, without giving rise to non-specific background. Opti buffer provides an optimal pH (7.8 - 8.0 at 70° C - 72° C) throughout the whole reaction, owing to a buffering capacity that is much higher than that of conventional Tris-HCl-based buffers. This is essential for the processivity and fidelity of thermostable DNA polymerases. Opti Buffer will enhance results with all templates, but is particularly useful for difficult templates and for the synthesis of long regions. Opti buffer is simply substituted in place of the incubation buffer supplied with thermostable polymerases, and provides the easiest way to improve the performance of DNA polymerase assays, without the user having to carry out time consuming optimization of the PCR conditions (Mg2+ template, dNTP and enzyme concentrations). Recommendations for use Prior to use, completely thaw Opti Buffer and vortex thoroughly. Storage Conditions Opti Buffer can be stored for 6 months at -20° C. Stock Solution Opti Buffer is supplied as a 10x solution.
DESCRIPTION Features and applications
PAN Mate is a special 2x additive for use in reactions involving any thermostable DNA polymerase, and is designed to dramatically improve reaction specificity. PAN Mate provides an optimized composition of reagents, and is ideally suited to dirty/difficult templates with GC or AT rich DNA, repetitive sequences or sequences with a high level of secondary structure. PAN Mate acts as a melting agent by allowing the DNA polymerase and oligonucleotides greater access to the template DNA. PAN Mate does not contain magnesium, dNTPs, or buffer components. In some cases it may be necessary to optimize the Magnesium concentration. Note: PAN Mate should not be used in combination with any other additives for polymerase reactions. PAN Mate is a revised version of PAN 5x HiSpec Additive (PAN737032). Storage Conditions PAN Mate additive can be stored for 6 months at -20° C.
DESCRIPTION Features and applications
PCR optimization kit facilitates successful PCR even with troublesome templates. The PCR optimisation kit consists of 12 ready-to-use 2x PCR Buffers (A-L), which are designed to rapidly determine the optimal conditions for all primer-template combinations. At least one of the 2x PCR Buffers will possess the optimal PCR conditions required to provide good results with your template. The kit dramatically reduces the time needed to set up reactions, and allows the user to optimize key variables without the need to test each variable independently. PCR optimization kit is ideal for use in difficult PCR , which often results from high GC or AT content, complex secondary structure, or in multiplex reactions. To ensure that the best possible results are achieved, a proprietary mix of enhancers is included in each 2x PCR Buffer. Once a specific PCR is optimized, individual PCR Buffers can be purchased separately. PCR optimization kit is suitable for templates of up to 5 Kb and delivers higher fidelity than Taq, since the active enzyme (PowerScript) possesses proofreading capability. PowerScript short DNA Polymerase PCR optimization kit is supplied with 100 Units of PowerScript short DNA Polymerase, a high-performance proprietary complex of enzymes specifically designed for difficult/problematic applications requiring high processivity and fidelity. 2x PCR optimization buffers 12 vials of buffer (A-L) contain dNTPs, variable concentration of MgCl2 (3 - 10 mM), and optimized composition of additives, PCR enhancers and reaction buffers. Storage Conditions PCR optimization kit can be stored for 6 months at -20° C.
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