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DESCRIPTION Features and applications
PAN DNA Clean is a novel, inexpensive solution, which provides a column-free method for nucleic-acid purification. Using a simple and rapid procedure, PAN DNA Clean can be used to purify or concentrate DNA or dsRNA from PCR reactions or any enzymatic digests. This method is easy to follow, combining convenience, speed and excellent recovery rates. Simple, Flexible and Column-free Protocol PAN DNA Clean removes proteins (such as restriction enzymes, polymerases, etc.), primers, primer-dimers and dNTPs. A very straightforward protocol allows the precipitation of nucleic acids ≥75 bp without the need for organic solvents, glass milk or expensive spin-columns. Unlike many column-based methods, PAN DNA Clean maximizes recovery with nucleic acid solutions, whether of low, medium or high concentration. PAN DNA Clean purifies nucleic acid without the use of chaotropic salts (which often contribute to denaturation of the DNA duplex). PAN DNA Clean enables the researcher to re-suspend the cleaned-up nucleic acids in any buffer and volume of choice, thus permitting the purification process to be tailored specifically to suit the experiment. Optimized Nucleic Acid Recovery PAN DNA Clean has been tailored to maximize the amount of nucleic acid recovered after purification, providing up to 98 % recovery of the original sample for immediate downstream applications, such as cloning and sequencing. PAN DNA Clean exhibits great versatility, achieving unsurpassed recovery rates, independently of the amount of nucleic acid or its concentration. Storage Conditions PAN DNA Clean solution can be stored at room temperature for 12 months. Do not freeze. Avoid exposure to light.
DESCRIPTION Features
PAN Dye Enhancer reduces the costs involved in auto-sequencing, by reducing the amount of dye-terminator needed in a reaction. Auto-sequencing reactions can leave up to 80 % of dye terminators unused, which normally require removal prior to sequencing. PAN Dye Enhancer works by providing optimal buffer conditions, which allow up to a 5-fold dilution of dye terminator without any loss of sequencing quality. PAN Dye Enhancer is suitable for sequencing of plasmid and PCR templates, and requires no optimization. For some templates it may be necessary to adjust the dilution factor. Storage Conditions PAN Dye Enhancer can be stored for 6 months at -20° C. Sample reactions using Half-Dye Mix. Reactions analyzed on ABI Prism 377 Auto-sequencer.
Plasmid Template: 1 in 3 dilution of big Dye terminator mix with PAN Dye Enhancer
PCR Template: 1 in 5 dilution of big Dye terminator mix with PAN Dye Enhancer
DESCRIPTION Features and applications
5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-GAL) is a chromogenic substrate for β-Galactosidase that forms an intense blue precipitate. It can be used in molecular biology to detect the gal gene product, and also in microbiology where it is used to detect micro-organisms which have β-Galactosidase activity (usually coliforms). It can be combined with the R-substrates to differentiate between two species of organisms on the same plate. X-GAL is soluble in N, N-dimethylformamide. Storage Conditions X-GAL can be stored for 12 months at -20° C. Store protected from light.
DESCRIPTION Features and applications
Isopropyl-β-D-thiogalactopyranoside (IPTG) is a chemical analogue of galactose, which cannot be hydrolysed by the enzyme β-Galactosidase. Hence, it induces the E. coli lac operon activity by binding and inhibiting the lac repressor without being degraded. Genes controlled by the lac or tac promoter/operator sequences are expressed to high levels in the presence of IPTG. Storage Conditions Store solid dry and protected from light. IPTG can be stored for 12 months at -20° C.
DESCRIPTION Features
Agarose (DNase/RNase-free) is an extremely pure, high molecular biology grade agarose powder that has been extensively tested for RNase contamination. Agarose provides high resolution of DNA and RNA separated by electrophoresis and offers consistent resolution from lot to lot. Storage Conditions Cool, dry place Analytical Specifications
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